Coding

Part:BBa_K4765011:Design

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)


MysB codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 532


Design Notes

We have codon-optimized the original sequence based on E. coli K12 to enable its expression in Escherichia coli.



Source

synthetic

References